Biotechnology services
Small Molecules Discovery Services
Large Molecules Discovery Services
Recombinant DNA Technology	Cloning and Vector Generation
	Ligation dependent and independent cloning
	Recombination based high throughput cloning
	De novo cloning (Synthetic gene fragments, PCR and cDNA based)
	Customized vector construction based on client requirement
Plasmid DNA Preparation	Research grade plasmid at small scale suitable for cloning, mutagenesis, microbial transformation and protein production.
	High quality industrial grade plasmid suitable for protein, antibody production, stable cell line generation in mammalian cells, bacmid generation for protein expression in insect cells, viral packaging, in vitro mRNA synthesis, vaccine, gene therapy studies and animal immunization.
	Process development for customized plasmid propagation and purification.
Mutagenesis	Customized mutagenesis specifications, including the creation of mutant libraries and site-directed and random mutagenesis.
	Point mutations, insertions, deletions, or substitutions of gene fragments.
	Alanine scanning, combinatorial mutagenesis, site saturation mutagenesis, and gene shuffle.
Antibody Gene Sequencing and Reformatting	RACE (Rapid Amplification of cDNA Ends) using template switching adapter ligation.
	Degenerate oligos.
	De novo antibody sequencing and analysis using LC-MS/MS.
	Reformatting antibody gene sequences into various other production formats like bispecifics, mAb, scFv, Fab, F (ab)’2, VHH/Nanobody.
	Antibody gene sequencing for Mouse, Rat, Rabbit and other species.
Strain Engineering	BSL-1 and BSL-2 microbes
	Gene knockout and knockin techniques based on homologous recombination.
	Characterization of engineered strains using microbiological methods, RT-PCR and advanced analytics.
	CRISPR-Cas9 based mutagenesis.
Gene Expression Analysis	Quantitative real time PCR.
	ELISA in various formats.
	Flow Cytometry.
	Western blotting.
	Mass Spectrometry.
Residual Protein, DNA and Mycoplasma Detection	Quantitative Real time PCR
	ELISA
	SPR (Biacore)
	Mycoplasma test
Protein Sciences: Protein Expression	Designing, scouting and screening studies for challenging proteins.
	Protein expression studies.
	With low running expenses, scale up using the spinner flask, shaker flask, HYPER flask, and WAVE bioreactor platforms to handle volumes up to 50 L scale.
Mammalian Expression Systems	Mammalian systems used for expression are CHO, ExpiCHO, HEK, ExpiHEK, etc.
	Protein production in transient expression mode or using stable clones or pools.
Insect Cell Expression Systems
Yeast Expression Systems
Bacterial Expression Systems	Soluble, periplasmic and inclusion bodies in E. coli
	Multi-protein expression by co-transfection or duet plasmid technology
	Refolding condition screening and method optimization
Protein Purification	Purification process optimization
	Automated FPLC systems and batch binding protein purification
	IMAC, IEC, mixed-mode, HIC, SEC, and affinity for micrograms to gram-scale quantities are chromatographic purification techniques.
Antibody Purification	Purification of monoclonal antibodies from various sources, including human and non-human primates, mice, rats, pigs, gerbils, and more.
	Generation from full-length mAbs and purification of various antibody fragments (Fab, Fab2, F(ab)’2, VHH, scfv, etc.)
	Bi-specific and KIH antibodies
	Diabodies
	Single domain antibody
	Protein purification from hybridoma cultures
Microbial Purification Capabilities	Soluble protein
	Periplasmic protein
	Intracellular protein
	Refolding from inclusion bodies
	Purification of Major Histocompatibility (MHC) complexes
	Membrane protein production – GPCRs, nanodisc technology, etc.
	Antibody drug conjugate screening, development and purification
	High throughput protein screening and purification
	Protein cleavage and purification
Protein Analytics and Characterization	Quantitative analysis: Protein estimation using Absorbance at 280nm,Bradford and BCA assays
	Qualitative analysis: SDS-PAGE (Reducing/Non-reducing) and DOT/Western blotanalysis
	Purity: SE-HPLC, IEX-HPLC and RP-HPLC
	Protein stability analysis (Real-time and Accelerated Stability Testing)
	LC-MS analysis
	Capillary electrophoresis (CE).
	Peptide Mass Fingerprinting (PMF)
	Biological activity and binding studies using enzymatic assays, ELISA, Biacore, etc.
	Endotoxin analysis
	Host cell protein (HCP) and Host cell DNA (HCD) analysis
Protein Stability Analysis	Real-time and accelerated stability testing.
	Long-term stability studies.
	Purity: SE-HPLC, IEX-HPLC and RP-HPLC
Protein Conjugation and Labelling	Protein labelling/conjugation (HRP, biotin, Ruthenium, fluorescent labels, etc.)
	Conjugation of resin with specific protein/peptide
	Miscellaneous based on customers requirement subject to Feasibility